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Literature summary extracted from

  • Almitairi, J.O.M.; Venkatraman Girija, U.; Furze, C.M.; Simpson-Gray, X.; Badakshi, F.; Marshall, J.E.; Schwaeble, W.J.; Mitchell, D.A.; Moody, P.C.E.; Wallis, R.
    Structure of the C1r-C1s interaction of the C1 complex of complement activation (2018), Proc. Natl. Acad. Sci. USA, 115, 768-773 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.41 expressed in Chinese hamster ovary cells Homo sapiens
3.4.21.42 expressed in Chinese hamster ovary cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.41 complex between the CUB1-EGF-CUB2 domains of C1r and C1s, sitting drop vapor diffusion method, in imidazole buffer at pH 8.0 with PEG 8000 as the precipitant Homo sapiens
3.4.21.42 complex between the CUB1-EGF-CUB2 domains of C1r and C1s, sitting drop vapor diffusion method, in imidazole buffer at pH 8.0 with PEG 8000 as the precipitant Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.41 Ca2+ dependent on Homo sapiens
3.4.21.42 Ca2+ dependent on Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.41 complement component C1s + H2O Homo sapiens
-
 activated complement component C1s + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.41 Homo sapiens P00736
-
-
3.4.21.42 Homo sapiens P09871
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.41 nickel-Sepharose affinity column chromatography and Superdex 200 gel filtration Homo sapiens
3.4.21.42 nickel-Sepharose affinity column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.41 complement component C1s + H2O
-
 Homo sapiens activated complement component C1s + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.41 homodimer 2 * 90000, component C1r, calculated from amino acid sequence Homo sapiens
3.4.21.42 homodimer 2 * 80000, component C1s, calculated from amino acid sequence Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.41 multiprotein complex C1 the multiprotein complex C1 is formed from the recognition subcomponent C1q and a tetramer of proteases C1r2C1s2 Homo sapiens
3.4.21.42 multiprotein complex C1 the multiprotein complex C1 is formed from the recognition subcomponent C1q and a tetramer of proteases C1r2C1s2 Homo sapiens

General Information

EC Number General Information Comment Organism
3.4.21.41 physiological function multiprotein complex C1 triggers the destruction of invading pathogens via lysis or by stimulation of innate and adaptive immune processes Homo sapiens
3.4.21.42 physiological function multiprotein complex C1 triggers the destruction of invading pathogens via lysis or by stimulation of innate and adaptive immune processes Homo sapiens